Anticorps Polyclonal de lapin anti-ZC3H12A

• Phare
• Validé par KD/KO

ZC3H12A Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 25009-1-AP

Synonymes

MCP induced protein 1, MCPIP, MCPIP1, Ribonuclease ZC3H12A, RP3 423B22.1, ZC3H12A

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Galerie de données de validation

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  WB analysis of K-562 using 25009-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 25009-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 25009-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 25009-1-AP

  IP result of anti-ZC3H12A (IP:25009-1-AP, 4ug; Detection:25009-1-AP 1:300) with Raji cells lysate 2000ug.

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  IHC staining of human small intestine using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25009-1-AP (ZC3H12A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25009-1-AP (ZC3H12A Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules Raji, tissu splénique de souris
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

25009-1-AP cible ZC3H12A dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZC3H12A Protéine recombinante Ag13877
• Nom complet: zinc finger CCCH-type containing 12A
• Masse moléculaire calculée: 599 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC005001
• Symbole du gène: ZC3H12A
• Identification du gène (NCBI): 80149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155.; Authors - Zhenzhen Li ; KD Validated
mouse	WB	Cell Physiol Biochem; Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.; Authors - Zhenzhen Li
mouse	IHC	J Immunol; MCPIP1/Regnase-1 Restricts IL-17A- and IL-17C-Dependent Skin Inflammation.; Authors - Leticia Monin
	WB	Retrovirology; Increased expression of CDKN1A/p21 in HIV-1 controllers is correlated with upregulation of ZC3H12A/MCPIP1.; Authors - Suwellen S D de Azevedo
mouse	IF	Neurosci Lett; TLR4 signaling pathway mediates the LPS/ischemia-induced expression of monocytechemotactic protein-induced protein 1 in microglia.; Authors - Shumin Chen
mouse	IF	Med Microbiol Immunol; MCPIP1 inhibits coxsackievirus B3 replication by targeting viral RNA and negatively regulates virus-induced inflammation.; Authors - Min Li