Anticorps Polyclonal de lapin anti-ZAP70

• Phare
• Validé par KD/KO

ZAP70 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 15592-1-AP

Synonymes

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

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Galerie de données de validation

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  WB analysis of Jurkat using 15592-1-AP

  WB result of ZAP70 antibody (15592-1-AP, 1:20000) with si-control and si-ZAP70 transfected Jurkat cells.

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  WB analysis using 15592-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15592-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15592-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15592-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15592-1-AP

  IP result of anti-ZAP70 (IP:15592-1-AP, 4ug; Detection:15592-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of RAW 264.7 using 15592-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using ZAP70 antibody (15592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A375
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de lymphome humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15592-1-AP cible ZAP70 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZAP70 Protéine recombinante Ag7943
• Nom complet: zeta-chain (TCR) associated protein kinase 70kDa
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC039039
• Symbole du gène: ZAP70
• Identification du gène (NCBI): 7535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Publications

Species	Application	Title
human	WB	J Immunol Methods; An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination; Authors - Jiaqi Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

hala (Verified Customer) (01-27-2023)

works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/10000
• Cell Tissue Type: hepG2

ZAP70 Antibody Western Blot validation (1/10000 dilution) in hepG2 (Cat no:15592-1-AP)