Anticorps Polyclonal de lapin anti-YWHAZ

• Phare
• Validé par KD/KO

YWHAZ Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 14881-1-AP

Synonymes

14 3 3 protein zeta/delta, 14-3-3, KCIP 1, YWHAZ

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Galerie de données de validation

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  WB analysis using 14881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14881-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Jurkat, cellules NIH/3T3, cellules Raji, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

14881-1-AP cible YWHAZ dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YWHAZ Protéine recombinante Ag6654
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC003623
• Symbole du gène: YWHAZ
• Identification du gène (NCBI): 7534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAZ.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB	EMBO J; AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.; Authors - Xin Zheng
human	WB,IHC	Oncogene; Lysine-specific demethylase 1 promotes the stemness and chemoresistance of Lgr5(+) liver cancer initiating cells by suppressing negative regulators of β-catenin signaling.; Authors - Z-J Lei ; KD Validated
		Oncogene; miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells.; Authors - X-H He
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong ; KD Validated
human	WB	Haematologica; Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.; Authors - Stephan Michalik

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erik (Verified Customer) (08-26-2021)

We used the antibody at 1:1000 dilution with 11ug input protein, and it produced one clean and strong band at the expected height for the protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MIAPACA2 and PANC1 cell lines

YWHAZ Antibody Western Blot validation (1:1000 dilution) in MIAPACA2 and PANC1 cell lines (Cat no:14881-1-AP)