Anticorps Polyclonal de lapin anti-YWHAH

• Phare
• Validé par KD/KO

YWHAH Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (2)

N° de cat : 15222-1-AP

Synonymes

14 3 3 protein eta, 14-3-3, 14-3-3 eta, Protein AS1, YWHA1, YWHAH

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Galerie de données de validation

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  WB analysis of human brain using 15222-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15222-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15222-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15222-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15222-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 15222-1-AP

  IP result of anti-YWHAH (IP:15222-1-AP, 4ug; Detection:15222-1-AP 1:1000) with NIH/3T3 cells lysate 1600ug.

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  IHC staining of mouse brain using 15222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15222-1-AP (YWHAH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15222-1-AP (YWHAH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15222-1-AP

  Immunofluorescent analysis of Hela cells, using YWHAH antibody 15222-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules A431, cellules NIH/3T3, cellules Raji, tissu cérébral de souris
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

15222-1-AP cible YWHAH dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YWHAH Protéine recombinante Ag7435
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC003047
• Symbole du gène: YWHAH
• Identification du gène (NCBI): 7533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η.; Authors - Jiarui Liu
human	WB,IF	Cell Biol Int; 14-3-3-η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage; Authors - Hui Zhou
rat	WB	Eur J Pharmacol; Capsaicin prevents mitochondrial damage, protects cardiomyocytes subjected to anoxia/reoxygenation injury mediated by 14-3-3η/Bcl-2.; Authors - Jiyi Huang
human,mouse	WB,IF,IP	J Exp Clin Cancer Res; 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.; Authors - Jia Song ; KD Validated
rat	WB	Cells; Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development; Authors - Nghi M Nguyen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vicki (Verified Customer) (01-13-2023)

Very expressive antibody used on BDEVs

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: EVs from Rat Brain

YWHAH Antibody Western Blot validation (1:2000 dilution) in EVs from Rat Brain (Cat no:15222-1-AP)

FH

Vicki (Verified Customer) (09-12-2022)

Works extremely well on hard to develop samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: EVs from Rat Brain

YWHAH Antibody Western Blot validation (1:1000 dilution) in EVs from Rat Brain (Cat no:15222-1-AP)