- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-XBP1S/XBP1U
XBP1S/XBP1U Polyclonal Antibody for WB, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (1)
Applications
WB, IHC, ChIP, ELISA
Conjugaison
Non conjugué
N° de cat : 24168-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HepG2, cellules HEK-293, cellules Jurkat, cellules Raji |
Résultats positifs en IHC | tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu hépatique de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 2 publications below |
WB | See 12 publications below |
IHC | See 2 publications below |
ChIP | See 1 publications below |
Informations sur le produit
24168-1-AP cible XBP1S/XBP1U dans les applications de WB, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | XBP1S/XBP1U Protéine recombinante Ag21454 |
Nom complet | X-box binding protein 1 |
Masse moléculaire calculée | 261 aa, 29 kDa |
Poids moléculaire observé | ~32 kDa; ~60 kDa |
Numéro d’acquisition GenBank | BC000938 |
Symbole du gène | XBP1 |
Identification du gène (NCBI) | 7494 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purifié par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.
Protocole
Product Specific Protocols | |
---|---|
WB protocol for XBP1S/XBP1U antibody 24168-1-AP | Download protocol |
IHC protocol for XBP1S/XBP1U antibody 24168-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Ethnopharmacol Network pharmacology, molecular docking, and in vitro experimental verification of the mechanism of Guanxining in treating diabetic atherosclerosis | ||
Bioorg Chem Dihydroartemisinin alleviates steatosis and inflammation in nonalcoholic steatohepatitis by decreasing endoplasmic reticulum stress and oxidative stress. | ||
J Virol Inositol requiring enzyme 1α promotes Zika virus infection through regulating stearoyl-CoA desaturase 1 mediated lipid metabolism.
| ||
J Zhejiang Univ Sci B Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure | ||
Cell Death Discov 24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death. | ||
J Exp Clin Cancer Res Calreticulin promotes EMT in pancreatic cancer via mediating Ca2+ dependent acute and chronic endoplasmic reticulum stress. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Carly (Verified Customer) (09-27-2019) | Used in an antibody array to identify proteins in human EDTA plasma.
|