Anticorps Polyclonal de lapin anti-WWP1

• Phare
• Validé par KD/KO

WWP1 Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 13587-1-AP

Synonymes

AIP5, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

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Galerie de données de validation

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  WB analysis of MCF-7 using 13587-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13587-1-AP (WWP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13587-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13587-1-AP (WWP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

13587-1-AP cible WWP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WWP1 Protéine recombinante Ag3582
• Nom complet: WW domain containing E3 ubiquitin protein ligase 1
• Masse moléculaire calculée: 922 aa, 105 kDa
• Poids moléculaire observé: 95-105 kDa
• Numéro d’acquisition GenBank: BC036065
• Symbole du gène: WWP1
• Identification du gène (NCBI): 11059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Elife; Sushi domain-containing protein 4 controls synaptic plasticity and motor learning.; Authors - Inés González-Calvo
human	WB,IHC	Oncotarget; Overexpression of WWP1 promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma.; Authors - Xiao-Fei Zhang ; KD Validated
human	WB	Mol Oncol; Protein kinase CK2 activation is required for transforming growth factor β-induced epithelial-mesenchymal transition.; Authors - Seongrak Kim
human	WB	Oncotarget; The eIF2α serine 51 phosphorylation-ATF4 arm promotes HIPPO signaling and cell death under oxidative stress.; Authors - Kamindla Rajesh
human, mouse	WB	J Biol Chem; E3 ubiquitin ligase, WWP1, interacts with AMPKα2 and down-regulates its expression in skeletal muscle C2C12 cells.; Authors - Lee Jung Ok JO
human	WB	Exp Cell Res; NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.; Authors - Jianning Xu