Anticorps Polyclonal de lapin anti-WSB2

• Phare
• Validé par KD/KO

WSB2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12124-2-AP

Synonymes

CS box containing WD protein, SBA2, WSB 2, WSB2

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Galerie de données de validation

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  WB analysis of PC-3 using 12124-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12124-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12124-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 12124-2-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12124-2-AP

  IP result of anti-WSB2 (IP:12124-2-AP, 3ug; Detection:12124-2-AP 1:800) with mouse kidney tissue lysate 6000ug.

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  IF Staining of HepG2 using 12124-2-AP

  Immunofluorescent analysis of HepG2 cells, using WSB2 antibody 12124-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules PC-3, tissu cérébral humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12124-2-AP cible WSB2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WSB2 Protéine recombinante Ag2768
• Nom complet: WD repeat and SOCS box-containing 2
• Masse moléculaire calculée: 404 aa, 45 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC015887
• Symbole du gène: WSB2
• Identification du gène (NCBI): 55884
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WSB2, also named SBA2, encodes WD repeat and SOCS box-containing protein 2. WSB2 may be a substrate-recognition component of an E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. WSB2 is a novel protein supposed to be involved in male- and female-specific gonadal development, and also a regulator of IL-21 receptor expression and IL21-induced signal transduction. WSB2 might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological functions need further studies.

Publications

Species	Application	Title
human	IHC	Cell Rep; Prognostic impact of vitamin B6 metabolism in lung cancer.; Authors - Galluzzi Lorenzo L
human	IP	Elife; Multiple cullin-associated E3 ligases regulate cyclin D1 protein stability; Authors - Ke Lu
	IHC	Oncoimmunology; Metabolic enzymes expressed by cancer cells impact the immune infiltrate.; Authors - Gautier Stoll
human	WB,IHC,CoIP	J Oncol; Retinoblastoma-Binding Protein 5 Regulates H3K4 Methylation Modification to Inhibit the Proliferation of Melanoma Cells by Inactivating the Wnt/β-Catenin and Epithelial-Mesenchymal Transition Pathways; Authors - Zhiqin Yang ; KD Validated