Anticorps Polyclonal de lapin anti-WNT5A/B

• Phare
• Validé par KD/KO

WNT5A/B Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

42

Avis (1)

N° de cat : 55184-1-AP

Synonymes

hWNT5A, Protein Wnt 5a, WNT5A, WNT5A B, WNT5A/B, WNT5B

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Galerie de données de validation

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  WB analysis using 55184-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55184-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 55184-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HUVEC, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu d'amygdalite humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 31 publications below
• IHC: See 6 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

55184-1-AP cible WNT5A/B dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: wingless-type MMTV integration site family, member 5A
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: NM_003392
• Symbole du gène: WNT5A
• Identification du gène (NCBI): 7474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides. This antibody can recognize WNT5A and WNT5B.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	IHC	Genome Biol; Integrated genomic and transcriptomic analysis reveals unique characteristics of hepatic metastases and pro-metastatic role of complement C1q in pancreatic ductal adenocarcinoma.; Authors - Jianyu Yang
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
		Biomaterials; Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.; Authors - Yonglin Yu
human	WB	Nanoscale; An assembly-inducing PDC enabling the efficient nuclear delivery of nucleic acid for cancer stem-like cell suppression; Authors - Dongyuan Wang
mouse	WB,IF,RIP	Arch Med Res; RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway; Authors - Zhao Yan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Darin (Verified Customer) (11-02-2017)

No signal in lung epithelial cells from mice. However, it is possible that the protein is not expressed in lung epithelial cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Lung

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