Anticorps Polyclonal de lapin anti-WNT4

WNT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14371-1-AP

Synonymes

WNT 4, UNQ426/PRO864, SERKAL, Protein Wnt-4, Protein Wnt 4

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Galerie de données de validation

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  WB analysis using 14371-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14371-1-AP (WNT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14371-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 14371-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules MCF-7,
• Résultats positifs en IHC: tissu rénal humain, tissu de cirrhose hépatique humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

14371-1-AP cible WNT4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT4 Protéine recombinante Ag5741
• Nom complet: wingless-type MMTV integration site family, member 4
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: BC057781
• Symbole du gène: WNT4
• Identification du gène (NCBI): 54361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WNT4 belongs to the Wnt family. WNT4 is critical for embryonic organogenesis, but later plays a unique role among Wnt proteins in the development and maintenance of müllerian and reproductive tissues (PMID: 33963381). The mesenchymal stem cells can improve cardiac function after cardiac injury by regulating endothelial cells via the Wnt4/β-catenin signaling pathway (PMID: 20551912). WNT4 may be modified by glycosylation, leading to an increase in molecular weight.

Publications

Species	Application	Title
human	WB	Endocrinology; PMID: 31748790; Authors - Alison M Neff
rat		Int J Clin Exp Pathol; Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1.; Authors - Junyi Hua
mouse	IHC	Neurochem Res; Wnt Signaling is Altered by Spinal Cord Neuronal Dysfunction in Amyotrophic Lateral Sclerosis Transgenic Mice.; Authors - Yu Li L
human	IF	Cell Death Dis; The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.; Authors - Nanchao Hong
mouse	IHC	BMC Complement Med Ther; Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathway.; Authors - Bing Cao
human	WB,IHC	Eur J Pharmacol; HucMSC exosomes attenuate partial bladder outlet obstruction-induced renal injury and cell proliferation via the Wnt/β-catenin pathway; Authors - Zhaoying Wang