Anticorps Polyclonal de lapin anti-HE4

HE4 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14406-1-AP

Synonymes

HE4, WAP5, WFDC2

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Galerie de données de validation

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  IHC staining of human ovary tumor using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14406-1-AP (HE4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14406-1-AP (HE4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14406-1-AP (HE4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14406-1-AP (HE4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14406-1-AP (HE4 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human testis using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14406-1-AP (HE4 antibody) at dilution of 1:25 (under 40x lens).

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  IF Staining of MCF-7 using 14406-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 14406-1-AP (HE4 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

14406-1-AP cible HE4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HE4 Protéine recombinante Ag5684
• Nom complet: WAP four-disulfide core domain 2
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: BC046106
• Symbole du gène: HE4
• Identification du gène (NCBI): 10406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

Publications

Species	Application	Title
		Biosens Bioelectron; An ultrasensitive ratiometric electrochemiluminescence immunosensor combining photothermal amplification for ovarian cancer marker detection.; Authors - Dandan Fang
mouse	WB	Oncotarget; Bcl-3 is a novel biomarker of renal fibrosis in chronic kidney disease.; Authors - Ran Chen
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang
human		RSC Adv; Machine learning algorithms enhance the specificity of cancer biomarker detection using SERS-based immunoassays in microfluidic chips; Authors - Nariman Banaei
human	IHC	Iran J Pathol; HE4, A New Potential Tumor Marker for Early Diagnosis and Predicting of Breast Cancer Progression.; Authors - Nazanin Mirmohseni Namini
human	IHC	Cell Rep; YAP targetome reveals activation of SPEM in gastric pre-neoplastic progression and regeneration; Authors - Adrian K H Loe