Anticorps Polyclonal de lapin anti-WDR61

WDR61 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 22536-1-AP

Synonymes

REC14, SKI8, WD repeat domain 61, WDR61

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Galerie de données de validation

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  WB analysis using 22536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22536-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 22536-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22536-1-AP

  IP result of anti-WDR61 (IP:22536-1-AP, 4ug; Detection:22536-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse testis using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22536-1-AP (WDR61 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22536-1-AP (WDR61 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22536-1-AP (WDR61 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22536-1-AP (WDR61 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 22536-1-AP

  Immunofluorescent analysis of HeLa cells using 22536-1-AP (WDR61 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

22536-1-AP cible WDR61 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR61 Protéine recombinante Ag18443
• Nom complet: WD repeat domain 61
• Masse moléculaire calculée: 305 aa, 34 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC010080
• Symbole du gène: WDR61
• Identification du gène (NCBI): 80349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency (refer to UniProt). Catalog#22536-1-AP is a rabbit polyclonal antibody raised against the full-length of human WDR61. The MW of this protein is 33 kDa, and this antibody specially recognises the 33 kDa protein.

Publications

Species	Application	Title
human	WB	Sci Adv; FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer; Authors - Yajie Liu
mouse	WB	Cell Death Dis; UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.; Authors - Gang Xiang
		Mol Cell; SPT6 functions in transcriptional pause/release via PAF1C recruitment; Authors - Yuki Aoi
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao