Anticorps Polyclonal de lapin anti-WDR18

• Phare
• Validé par KD/KO

WDR18 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15165-1-AP

Synonymes

R32184_1, WD repeat domain 18, WDR18

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Galerie de données de validation

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  WB analysis of Jurkat using 15165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15165-1-AP (WDR18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15165-1-AP (WDR18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15165-1-AP (WDR18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15165-1-AP (WDR18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15165-1-AP (WDR18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15165-1-AP (WDR18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15165-1-AP (WDR18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15165-1-AP (WDR18 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat
• Résultats positifs en IHC: tissu pulmonaire humain, tissu de cancer du poumon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

15165-1-AP cible WDR18 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR18 Protéine recombinante Ag7282
• Nom complet: WD repeat domain 18
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC001648
• Symbole du gène: WDR18
• Identification du gène (NCBI): 57418
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WDR18 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit. This complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation (PMID:21326211).

Publications

Species	Application	Title
human	WB	EMBO J; The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.; Authors - Finkbeiner Elisabeth E ; KD Validated
human	WB	Mol Cell Proteomics; Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.; Authors - Fanis Pavlos P
human	WB	PLoS One; A Role of hIPI3 in DNA Replication Licensing in Human Cells.; Authors - Yining Huang
human	IP,WB	Mol Oncol; PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis; Authors - Xue Yang