Anticorps Monoclonal anti-WASF3

WASF3 Monoclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

3B7D10

N° de cat : 67620-1-Ig

Synonymes

Brush 1, KIAA0900, Protein WAVE 3, SCAR3, WAS protein family, member 3, WASF3, WASP family protein member 3, WAVE3

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Galerie de données de validation

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  WB analysis using 67620-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67620-1-Ig (WASF3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67620-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67620-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67620-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67620-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67620-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 67620-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris, tissu de cancer du foie humain

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67620-1-Ig cible WASF3 dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WASF3 Protéine recombinante Ag29991
• Nom complet: WAS protein family, member 3
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50-55 and 66-70 kDa
• Numéro d’acquisition GenBank: BC050283
• Symbole du gène: WASF3
• Identification du gène (NCBI): 10810
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WASF3, also named WAVE3 or SCAR3, belongs to the SCAR/WAVE family. It plays a role in the regulation of cell morphology and cytoskeletal organization. WASF3 is associated with movement, invasion, and metastasis in different cancers such as breast cancer, gastric cancer, and prostate cancer. It has been shown that WASF3 levels are elevated in advanced-stage cancers compared to lower-grade tumors and normal tissue. The 67620-1-Ig antibody recognizes 50-55 kDa and 66-70 kDa proteins similar to the papers.(PMID: 21544801, 15826941, 31038356, 27432794, 31542393)