Anticorps Polyclonal de lapin anti-VTI1A

VTI1A Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12354-1-AP

Synonymes

MVti1, Vti1 rp2, VTI1A

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Galerie de données de validation

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  WB analysis of human brain using 12354-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12354-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12354-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12354-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12354-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12354-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 12354-1-AP

  IP result of anti-VTI1A (IP:12354-1-AP, 3ug; Detection:12354-1-AP 1:1000) with MCF-7 cells lysate 520ug.

• 

  IF Staining of NIH/3T3 using 12354-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 12354-1-AP (VTI1A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules MCF-7, tissu cardiaque humain, tissu cérébral de souris, tissu pulmonaire humain, tissu rénal humain
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IF/ICC: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

12354-1-AP cible VTI1A dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VTI1A Protéine recombinante Ag3018
• Nom complet: vesicle transport through interaction with t-SNAREs homolog 1A (yeast)
• Masse moléculaire calculée: 217 aa, 25 kDa
• Poids moléculaire observé: 25-29 kDa
• Numéro d’acquisition GenBank: BC017052
• Symbole du gène: VTI1A
• Identification du gène (NCBI): 143187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). VTI1A is involved in transport between the endosome and the trans-Golgi network. The SNARE complex of STX10, STX16, VTI1A, and VAMP3 is required for MPR (mannose 6-phosphate receptors) transport from endosomes to the Golgi after delivering lysosomal enzymes to the endocytic pathway (PMID: 18195106). Transport from early/recycling endosomes to the TGN is mediated by the SNARE complex of STX6, STX16, VTI1A and VAMP4 (PMID: 11839770; 21807881).

Publications

Species	Application	Title
human	WB,IF	J Cell Biol; The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.; Authors - Laufman Orly O
human	WB,IF	J Cell Sci; The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.; Authors - Laufman Orly O
human	WB	Oncotarget; Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.; Authors - Yi Huo
human	WB,IF	Traffic; Deficiency of the Cog8 subunit in normal and CDG-derived cells impairs the assembly of the COG and Golgi SNARE complexes.; Authors - Laufman Orly O
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik
mouse	WB	Proc Natl Acad Sci U S A; Nanoelectrochemistry reveals how soluble Aβ42 oligomers alter vesicular storage and release of glutamate; Authors - Xiao-Ke Yang