Anticorps Polyclonal de lapin anti-VPS8

• Phare
• Validé par KD/KO

VPS8 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15079-1-AP

Synonymes

KIAA0804, VPS8

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Galerie de données de validation

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  WB analysis of human brain using 15079-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15079-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15079-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15079-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15079-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 15079-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15079-1-AP

  IP result of anti-VPS8 (IP:15079-1-AP, 4ug; Detection:15079-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IF Staining of HepG2 using 15079-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15079-1-AP (VPS8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules PC-3, tissu cérébral de souris, tissu hépatique de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

15079-1-AP cible VPS8 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS8 Protéine recombinante Ag7151
• Nom complet: vacuolar protein sorting 8 homolog (S. cerevisiae)
• Masse moléculaire calculée: 162 kDa
• Poids moléculaire observé: 151 kDa
• Numéro d’acquisition GenBank: BC001001
• Symbole du gène: VPS8
• Identification du gène (NCBI): 23355
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS8, also named as KIAA0804, has some isoforms with MW 135-160 kDa and 70-90 kDa.

Publications

Species	Application	Title
human	WB	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang ; KD Validated
human	WB, IP	Traffic; Mammalian CORVET is required for fusion and conversion of distinct early endosome sub-populations.; Authors - Enrico Daniele Perini
human	WB	Biochem J; VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.; Authors - Morag R Hunter
human	WB	Sci Rep; HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis; Authors - Seigo Terawaki ; KO Validated