Anticorps Polyclonal de lapin anti-VPS72

• Phare
• Validé par KD/KO

VPS72 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15143-1-AP

Synonymes

CFL1, Protein YL 1, Swc2, TCFL1, Transcription factor like 1, VPS72, YL 1, YL1

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Galerie de données de validation

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  WB analysis of HeLa using 15143-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15143-1-AP

  WB result of VPS72 antibody (15143-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS72 transfected A549 cells.

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  WB analysis of NIH/3T3 using 15143-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15143-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 15143-1-AP

  IP result of anti-VPS72 (IP:15143-1-AP, 4ug; Detection:15143-1-AP 1:500) with NIH/3T3 cells lysate 4000 ug.

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  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules NIH/3T3, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IHC: tissu d'hyperplasie mammaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15143-1-AP cible VPS72 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS72 Protéine recombinante Ag7424
• Nom complet: vacuolar protein sorting 72 homolog (S. cerevisiae)
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC003151
• Symbole du gène: VPS72
• Identification du gène (NCBI): 6944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian TRRAP/TIP60-containing histone acetyltransferase (HAT) complex, which exists in Drosophila melanogaster and mammalian cells, is a complex that is responsible for various cellular processes, including DNA repair, transcriptional activation and apoptosis. Vacuolar protein sorting-associated protein 72 homolog (VPS72), also known as VPS72 or Transcription factor-like 1, is a 364 amino acid subunit of the TRRAP/TIP60 HAT complex. VPS72 has also been identified as a subunit of a novel complex containing SNF2-related helicase SRCAP (SWI2/SNF2-related CBP activator protein). This SRCAP-containing complex is very similar to the S. cerevisiae SWR1 chromatin remodeling complex. The involvement of VPS72 in these complexes has suggested that VPS72 plays multiple roles in chromatin modification and remodeling in cells. VPS72 localizes to the nucleus and is phosphorylated upon DNA damage, most likely by ATM or ATR.

Publications

Species	Application	Title
		Mol Cell; PMID: 28366643; Authors - Sophie Barral
	WB	Cell Discov; Structural insights into histone exchange by human SRCAP complex; Authors - Jiali Yu