Anticorps Polyclonal de lapin anti-VPS54

• Phare
• Validé par KD/KO

VPS54 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13327-1-AP

Synonymes

HCC8, hVps54L, SLP 8p, Tumor antigen HOM HCC 8, Tumor antigen SLP 8p, VPS54, VPS54L

---

Galerie de données de validation

• 

  WB analysis of human brain using 13327-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13327-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13327-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13327-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13327-1-AP

  IP result of anti-VPS54 (IP:13327-1-AP, 4ug; Detection:13327-1-AP 1:300) with mouse brain tissue lysate 4000ug.

---

Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

13327-1-AP cible VPS54 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS54 Protéine recombinante Ag4123
• Nom complet: vacuolar protein sorting 54 homolog (S. cerevisiae)
• Masse moléculaire calculée: 977 aa, 111 kDa
• Poids moléculaire observé: 111 kDa
• Numéro d’acquisition GenBank: BC030275
• Symbole du gène: VPS54
• Identification du gène (NCBI): 51542
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.

Publications

Species	Application	Title
mouse	WB	Cell Rep; The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.; Authors - Jian Wei
mouse	WB	J Biol Chem; TDP-43 and FUS RNA-binding proteins bind distinct sets of cytoplasmic messenger RNAs and differently regulate their post-transcriptional fate in motoneuron-like cells.; Authors - Colombrita Claudia C
human	WB	Cancer Res Commun; Repurposing Itraconazole and Hydroxychloroquine to Target Lysosomal Homeostasis in Epithelial Ovarian Cancer; Authors - Stefano Marastoni ; KD Validated