Anticorps Polyclonal de lapin anti-VPS45

VPS45 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12616-1-AP

Synonymes

h VPS45, H1, H1VPS45, hlVps45, VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A

---

Galerie de données de validation

• 

  WB analysis of mouse liver using 12616-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12616-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12616-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12616-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12616-1-AP

  IP result of anti-VPS45 (IP:12616-1-AP, 4ug; Detection:12616-1-AP 1:300) with mouse kidney tissue lysate 3000 ug.

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu cérébral de souris, tissu pancréatique de rat, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12616-1-AP cible VPS45 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS45 Protéine recombinante Ag3289
• Nom complet: vacuolar protein sorting 45 homolog (S. cerevisiae)
• Masse moléculaire calculée: 570 aa, 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC028382
• Symbole du gène: VPS45
• Identification du gène (NCBI): 11311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

Publications

Species	Application	Title
human	WB	J Cell Sci; The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.; Authors - Laufman Orly O