Anticorps Polyclonal de lapin anti-VPS26B

VPS26B Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 15915-1-AP

Synonymes

Pep8b, Vesicle protein sorting 26B, VPS26B

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Galerie de données de validation

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  WB analysis using 15915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15915-1-AP (VPS26B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15915-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15915-1-AP (VPS26B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15915-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15915-1-AP (VPS26B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15915-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15915-1-AP (VPS26B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15915-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS26B antibody (15915-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15915-1-AP cible VPS26B dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS26B Protéine recombinante Ag8708
• Nom complet: vacuolar protein sorting 26 homolog B (S. pombe)
• Masse moléculaire calculée: 336 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC009747
• Symbole du gène: VPS26B
• Identification du gène (NCBI): 112936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In mammals, there are two paralogues of yeast Vps26, VPS26A and VPS26B (PMID: 16190980). VPS26 is a component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. VPS26A and VPS26B subunits define distinct retromer complexes (PMID: 21920005). The retromer complex is important in recycling transmembrane receptors from endosomes to the trans-Golgi network (TGN).

Publications

Species	Application	Title
mouse	WB	Mol Psychiatry; Full recovery of the Alzheimer's disease phenotype by gain of function of vacuolar protein sorting 35.; Authors - Jian-Guo Li
mouse	WB	Nat Commun; Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis.; Authors - Luca Muzio
human	WB	Mol Psychiatry; VPS35 regulates tau phosphorylation and neuropathology in tauopathy.; Authors - Alana N Vagnozzi
rat	WB,CoIP,IF	Cell Death Differ; Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.; Authors - Jing Lu
human	WB, IP	Proc Natl Acad Sci U S A; A unique PDZ domain and arrestin-like fold interaction reveals mechanistic details of endocytic recycling by SNX27-retromer.; Authors - Matthew Gallon
human	WB	Ann Neurol; Dysregulation of the Retromer Complex System in Down Syndrome.; Authors - Mary Elizabeth Curtis

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xin (Verified Customer) (10-10-2022)

It is reliable to detect VPS26B in mouse brain lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

VPS26B Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:15915-1-AP)