Anticorps Polyclonal de lapin anti-VPRBP

• Phare
• Validé par KD/KO

VPRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

40

Avis (2)

N° de cat : 11612-1-AP

Synonymes

KIAA0800, HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 11612-1-AP

  WB result of VPRBP antibody (11612-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells.

• 

  WB analysis using 11612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11612-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11612-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11612-1-AP

  IP result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug.

• 

  IHC staining of human prostate cancer using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11612-1-AP (VPRBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11612-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (11612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 11612-1-AP

  1X10^6 HeLa cells were stained with 0.2ug VPRBP antibody (11612-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562, cellules PC-3, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 19 publications below
• WB: See 36 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 4 publications below

Informations sur le produit

11612-1-AP cible VPRBP dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPRBP Protéine recombinante Ag2184
• Nom complet: Vpr (HIV-1) binding protein
• Masse moléculaire calculée: 1506 aa, 169 kDa
• Poids moléculaire observé: 169 kDa
• Numéro d’acquisition GenBank: BC022792
• Symbole du gène: VPRBP
• Identification du gène (NCBI): 9730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

Publications

Species	Application	Title
human	WB,IHC,IF	Science; CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.; Authors - Chao Yu ; KD Validated
mouse, human	WB, IF	Mol Cell; CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases.; Authors - Tadashi Nakagawa ; KD Validated
human	WB	Mol Cell; Vpr Targets TET2 for Degradation by CRL4VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication.; Authors - Lei Lv ; KD Validated
human	WB	Nat Microbiol; Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.; Authors - Leonid Yurkovetskiy
human	WB	Cell Host Microbe; Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production.; Authors - Michael Mashiba
human	WB, IF	Cell Host Microbe; Cyclin L2 Is a Critical HIV Dependency Factor in Macrophages that Controls SAMHD1 Abundance.; Authors - George Boateng Kyei ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Roy (Verified Customer) (06-13-2024)

Great antibody detecting DCAF1 (VPRBP) validated in our coIP/WB experiments (1/1000 diluation - 1h incubation at RT)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa cells

FH

Nikhil (Verified Customer) (09-17-2019)

Very good for both IP and western blot.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Colon cancer cells

VPRBP Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in Colon cancer cells (Cat no:11612-1-AP)

Produits associés

CUL4A-Specific Polyclonal antibody

14851-1-AP

RBX1 Polyclonal antibody

14895-1-AP

DDB1 Polyclonal antibody

11380-1-AP

SAMHD1 Polyclonal antibody

12586-1-AP

Neurofibromin 2 Polyclonal antibody

21686-1-AP

DDA1 Polyclonal antibody

14995-1-AP