Anticorps Polyclonal de lapin anti-VIP

VIP Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 16233-1-AP

Synonymes

VIP peptides, Intestinal peptide PHV-42, Intestinal peptide PHM-27

---

Galerie de données de validation

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 16233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using VIP antibody (16233-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Calretinin antibody (66496-1-Ig, Clone: 2D7A9, red).

• 

  IF Staining of mouse colon using 16233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using VIP antibody (16233-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Calretinin antibody (66496-1-Ig, Clone: 2D7A9, red).

• 

  IF Staining of COLO 320 using 16233-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 16233-1-AP (VIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en IHC: tissu de côlon humain, tissu cérébral de souris, tissu d'intestin grêle de rat, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris,
• Résultats positifs en IF/ICC: cellules COLO 320,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

16233-1-AP cible VIP dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VIP Protéine recombinante Ag8933
• Nom complet: vasoactive intestinal peptide
• Masse moléculaire calculée: 169 aa, 19 kDa
• Numéro d’acquisition GenBank: BC009794
• Symbole du gène: VIP
• Identification du gène (NCBI): 7432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vasoactive intestinal peptide (VIP), a short peptide containing 28 amino acids belonging to the secretin-glucagon family, is initially isolated from the gastrointestinal tract as a potent vasodilator peptide. VIP was initially identified in normal nervous tissue and neurons and was subsequently recognized as a neurotransmitter widely distributed in various tissues. The wide distribution of VIP determines its involvement in a range of biological activities, such as gut motility, hormonal regulation, circadian rhythms, immune responses, and carcinogenesis. The general physiologic effects of VIP include vasodilation, anti-inflammatory actions, cell proliferation, hormonal secretion, regulation of gastric motility, and smooth muscle relaxation; therefore, VIP has emerged as a promising drug candidate for the treatment of several diseases.

Publications

Species	Application	Title
mouse	IHC	Immun Inflamm Dis; Vasoactive intestinal peptide exerts therapeutic action by regulating PTEN in a model of Sjögren's disease; Authors - Yixi Li
mouse	IF	Pharm Biol; Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway; Authors - Muhua Huang
mouse	IF	Cell Rep; PMID: 31509750; Authors - Jing Xue
rat	WB	Biomolecules; Postweaning Development Influences Endogenous VPAC1 Modulation of LTP Induced by Theta-Burst Stimulation: A Link to Maturation of the Hippocampal GABAergic System; Authors - Marta Gil

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Reyes (Verified Customer) (03-01-2024)

VIP (in red) worked nicely on IF in human paraffin brain sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

VIP Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:16233-1-AP)