Anticorps Polyclonal de lapin anti-VHL

VHL Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 24756-1-AP

Synonymes

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1

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Galerie de données de validation

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  WB analysis using 24756-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24756-1-AP (VHL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24756-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24756-1-AP (VHL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24756-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24756-1-AP (VHL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 24756-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24756-1-AP (VHL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24756-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24756-1-AP (VHL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24756-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24756-1-AP (VHL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24756-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24756-1-AP (VHL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 24756-1-AP

  Immunofluorescent analysis of HepG2 cells using 24756-1-AP (VHL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules Raji
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

24756-1-AP cible VHL dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VHL Protéine recombinante Ag21447
• Nom complet: von Hippel-Lindau tumor suppressor
• Masse moléculaire calculée: 172 aa, 20 kDa
• Poids moléculaire observé: 18-24 kDa
• Numéro d’acquisition GenBank: BC058831
• Symbole du gène: VHL
• Identification du gène (NCBI): 7428
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

Publications

Species	Application	Title
human	WB	Front Oncol; Hypoxia-Induced LncRNA-MIR210HG Promotes Cancer Progression By Inhibiting HIF-1α Degradation in Ovarian Cancer.; Authors - Ping Liu
human	WB	Microvasc Res; A positive feedback loop between miR-574-3p and HIF-1α in promoting angiogenesis under hypoxia; Authors - Renwen Zhang
	CoIP	Cell Death Discov; Septin4 promotes cardiomyocytes apoptosis by enhancing the VHL-mediated degradation of HIF-1α.; Authors - Shaojun Wu
human	WB,CoIP	Pathol Oncol Res; MDM2 promotes cancer cell survival through regulating the expression of HIF-1α and pVHL in retinoblastoma; Authors - Shouhua Zhang
human	WB	EMBO J; A mitochondrial EglN1-AMPKα axis drives breast cancer progression by enhancing metabolic adaptation to hypoxic stress; Authors - Weiwei Jiang
human	IHC,WB	Cancer Res; Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression; Authors - Lijie Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (11-22-2021)

This antibody didn't work in my hands. There are too many non-specific bands to identify the VHL band.

• Applications: Western Blot

VHL Antibody Western Blot validation ( dilution) in (Cat no:24756-1-AP)