Anticorps Polyclonal de lapin anti-VHL

VHL Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (3)

N° de cat : 16538-1-AP

Synonymes

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1, Von Hippe-Lindau

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Galerie de données de validation

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  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16538-1-AP (VHL antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16538-1-AP (VHL antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:100 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:100 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HepG2 using 16538-1-AP

  Immunofluorescent analysis of HepG2 cells, using VHL antibody 16538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

16538-1-AP cible VHL dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VHL Protéine recombinante Ag9843
• Nom complet: von Hippel-Lindau tumor suppressor
• Masse moléculaire calculée: 172 aa, 20 kDa
• Numéro d’acquisition GenBank: BC058831
• Symbole du gène: VHL
• Identification du gène (NCBI): 7428
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

Publications

Species	Application	Title
human,mouse	IHC	Respir Res; Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin.; Authors - Changhui Yu
human		Oncotarget; The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.; Authors - Fabao Liu
human	IHC	Front Oncol; Risk Scores Based on Six Survival-Related RNAs in a Competing Endogenous Network Composed of Differentially Expressed RNAs Between Clear Cell Renal Cell Carcinoma Patients Carrying Wild-Type or Mutant Von Hippel-Lindau Serve Well to Predict Malignancy and Prognosis.; Authors - Rui Zhu
human,mouse	IHC	Front Mol Biosci; Network-Based Expression Analyses and Experimental Verifications Reveal the Involvement of STUB1 in Acute Kidney Injury.; Authors - Yanting Shi
human,mouse	IHC,IF	Cancer Med; UBE2S promotes malignant properties via VHL/HIF-1α and VHL/JAK2/STAT3 signaling pathways and decreases sensitivity to sorafenib in hepatocellular carcinoma; Authors - Junyi Wu
human		World J Gastroenterol; Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways; Authors - Zhan-Xue Zhao ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jingwen (Verified Customer) (01-27-2020)

This antibody worked very well on the cancer samples by performing western blot and immunofluorescence.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Cancer tissue

FH

Jie (Verified Customer) (01-27-2020)

achieved positive staining in liver cancer cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:200

FH

Lunfeng (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,