Anticorps Polyclonal de lapin anti-VAV1

• Phare
• Validé par KD/KO

VAV1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16364-1-AP

Synonymes

Proto oncogene vav, VAV, VAV1

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Galerie de données de validation

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  WB analysis of Jurkat using 16364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16364-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16364-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16364-1-AP

  IP result of anti-VAV1 (IP:16364-1-AP, 4ug; Detection:16364-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human breast cancer using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque humain, tissu cérébral humain, tissu de lymphome humain, tissu hépatique humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

16364-1-AP cible VAV1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, Gecko
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAV1 Protéine recombinante Ag9049
• Nom complet: vav 1 guanine nucleotide exchange factor
• Masse moléculaire calculée: 845 aa, 98 kDa
• Poids moléculaire observé: 98 kDa
• Numéro d’acquisition GenBank: BC013361
• Symbole du gène: VAV1
• Identification du gène (NCBI): 7409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
gecko	WB,IF	J Biol Chem; Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.; Authors - Nan Du ; KD Validated
human	WB,IHC	Biochem Biophys Res Commun; Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis.; Authors - Xiaolei Zhu
human	WB	Oncotarget; Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.; Authors - Wei-Zhen Xue
rat	WB,IF	Clin Sci (Lond); Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.; Authors - Chang-Yien Chan ; KD Validated
human	WB	Cancer Cell Int; STING pathway contributes to the prognosis of hepatocellular carcinoma and identification of prognostic gene signatures correlated to tumor microenvironment; Authors - Zhangya Pu
rat	IF	Neural Regen Res; Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation; Authors - Jing Qiu