Anticorps Polyclonal de lapin anti-VARS2

• Phare
• Validé par KD/KO

VARS2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15776-1-AP

Synonymes

KIAA1885, Valine tRNA ligase, ValRS, Valyl tRNA synthetase like, VARS2, VARS2L, VARSL

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Galerie de données de validation

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  WB analysis using 15776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15776-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15776-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15776-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15776-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15776-1-AP

  IP result of anti-VARS2 (IP:15776-1-AP, 4ug; Detection:15776-1-AP 1:500) with HeLa cells lysate 2400ug.

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  IHC staining of human liver cancer using 15776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15776-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15776-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15776-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15776-1-AP (VARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cérébral humain, tissu hépatique humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15776-1-AP cible VARS2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VARS2 Protéine recombinante Ag8460
• Nom complet: valyl-tRNA synthetase 2, mitochondrial (putative)
• Masse moléculaire calculée: 119 kDa, 57 kDa
• Poids moléculaire observé: 118 kDa
• Numéro d’acquisition GenBank: BC008844
• Symbole du gène: VARS2
• Identification du gène (NCBI): 57176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.

Publications

Species	Application	Title
human	IF	EMBO Mol Med; The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.; Authors - Elena Perli
human	IHC	Int J Mol Sci; VARS2 Depletion Leads to Activation of the Integrated Stress Response and Disruptions in Mitochondrial Fatty Acid Oxidation.; Authors - Elham Kayvanpour ; KD Validated
human	WB	Front Pediatr; Case Report and Review of the Literature: A New and a Recurrent Variant in the VARS2 Gene Are Associated With Isolated Lethal Hypertrophic Cardiomyopathy, Hyperlactatemia, and Pulmonary Hypertension in Early Infancy.; Authors - Katarína Kušíková
human	WB	Metab Brain Dis; Neonatal encephalocardiomyopathy caused by mutations in VARS2.; Authors - Fabian Baertling