Anticorps Polyclonal de lapin anti-VAMP8

VAMP8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 15546-1-AP

Synonymes

EDB, Endobrevin, VAMP 8, VAMP8

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Galerie de données de validation

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  WB analysis using 15546-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 15546-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules PC-12, cellules THP-1
• Résultats positifs en IHC: tissu placentaire humain, tissu placentaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

15546-1-AP cible VAMP8 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAMP8 Protéine recombinante Ag7903
• Nom complet: vesicle-associated membrane protein 8 (endobrevin)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC001634
• Symbole du gène: VAMP8
• Identification du gène (NCBI): 8673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.

Publications

Species	Application	Title
human	IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
rat	WB	Arch Toxicol; Mystery of methamphetamine-induced autophagosome accumulation in hippocampal neurons: loss of syntaxin 17 in defects of dynein-dynactin driving and autophagosome-late endosome/lysosome fusion.; Authors - Huaisha Xu
human	WB	Ecotoxicol Environ Saf; Weakened interaction of ATG14 and the SNARE complex blocks autophagosome-lysosome fusion contributes to fluoride-induced developmental neurotoxicity.; Authors - Yuanli Zhang
rat	WB	Stem Cell Res Ther; Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells.; Authors - Zheng Li
human	WB	Nutrients; Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.; Authors - Hongkun Lin