Anticorps Polyclonal de lapin anti-VAMP7/TI-VAMP

• Phare
• Validé par KD/KO

VAMP7/TI-VAMP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA, Blocking

Conjugaison

Non conjugué

Publications

5

N° de cat : 22268-1-AP

Synonymes

VAMP7, TI VAMP, Tetanus-insensitive VAMP, Tetanus insensitive VAMP, Synaptobrevin-like protein 1

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Galerie de données de validation

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  WB analysis using 22268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22268-1-AP

  IP result of anti-VAMP7/TI-VAMP (IP:22268-1-AP, 4ug; Detection:22268-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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  IHC staining of human liver using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 22268-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAMP7/TI-VAMP antibody (22268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 22268-1-AP

  Immunofluorescent analysis of HepG2 cells using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

22268-1-AP cible VAMP7/TI-VAMP dans les applications de WB, IHC, IF/ICC, IP, ELISA, Blocking et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: vesicle-associated membrane protein 7
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: NM_005638
• Symbole du gène: VAMP7
• Identification du gène (NCBI): 6845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VAMP7, also named as SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is required for calcium regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.

Publications

Species	Application	Title
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan ; KO Validated
human	WB	Cancer Res Commun; Combination of a New Oral Demethylating Agent, OR2100, and Venetoclax for Treatment of Acute Myeloid Leukemia; Authors - Kazuharu Kamachi ; KD Validated
mouse	WB,IF	Biochem Biophys Res Commun; VAMP7 knockdown in secretory granules impairs CCL2 secretion in mast cells; Authors - Marin Sakamoto ; KD Validated
rat	WB	Small; Key Role of Microtubule and Its Acetylation in a Zinc Oxide Nanoparticle-Mediated Lysosome-Autophagy System.; Authors - Jia Liu