Anticorps Monoclonal anti-VAMP4

VAMP4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A6F2

N° de cat : 67219-1-PBS

Synonymes

3A6F2, VAMP-4, Vesicle-associated membrane protein 4

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Galerie de données de validation

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  WB analysis using 67219-1-Ig (same clone as 67219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67219-1-Ig (VAMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67219-1-Ig (VAMP4 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67219-1-PBS cible VAMP4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VAMP4 Protéine recombinante Ag27854
• Nom complet: vesicle-associated membrane protein 4
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC007019
• Symbole du gène: VAMP4
• Identification du gène (NCBI): 8674
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

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