Anticorps Polyclonal de lapin anti-VAMP4

• Phare
• Validé par KD/KO

VAMP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 10738-1-AP

Synonymes

Vesicle-associated membrane protein 4, VAMP-4

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Galerie de données de validation

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  WB analysis of mouse testis using 10738-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 10738-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10738-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of SH-SY5Y using 10738-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10738-1-AP

  IP result of anti-VAMP4 (IP:10738-1-AP, 4ug; Detection:10738-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 10738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10738-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10738-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 10738-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 10738-1-AP (VAMP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10738-1-AP cible VAMP4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAMP4 Protéine recombinante Ag1090
• Nom complet: vesicle-associated membrane protein 4
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC007019
• Symbole du gène: VAMP4
• Identification du gène (NCBI): 8674
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

Publications

Species	Application	Title
mouse,rat	WB,IF,IP	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li ; KO Validated
human	WB	J Biol Chem; Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.; Authors - Wulin Aerbajinai
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik
rat	WB	Biophys Rep; PMID: 30596141; Authors - Min Li
mouse	IF	J Biol Chem; Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.; Authors - Tao Tao
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priyadarshini (Verified Customer) (04-30-2020)

The antibody works reliably for immuno-fluorescent staining of primary neuron cultures from rat embryos.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:75
• Cell Tissue Type: Primary hippocampal neurons (Rat E17)

VAMP4 Antibody Immunofluorescence validation (1:75 dilution) in Primary hippocampal neurons (Rat E17) (Cat no:10738-1-AP)