Anticorps Polyclonal de lapin anti-VAMP2

VAMP2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA, Blocking

Conjugaison

Non conjugué

Publications

25

N° de cat : 10135-1-AP

Synonymes

SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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Galerie de données de validation

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  WB analysis using 10135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10135-1-AP (VAMP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 10135-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10135-1-AP (VAMP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10135-1-AP (VAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10135-1-AP (VAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10135-1-AP (VAMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10135-1-AP (VAMP2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu cérébral de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 12 publications below

Informations sur le produit

10135-1-AP cible VAMP2 dans les applications de WB, IF, IHC, ELISA, Blocking et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAMP2 Protéine recombinante Ag0179
• Nom complet: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC002737
• Symbole du gène: VAMP2
• Identification du gène (NCBI): 6844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
human	WB,IF	Adv Sci (Weinh); Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage; Authors - Nikita Raj
	IF	Neuron; Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.; Authors - Roberta Cagnetta
mouse	IHC,IF	Brain; Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.; Authors - Lina Pan
human	IF	Sci Signal; A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity; Authors - Hyunbae Kim