Anticorps Polyclonal de lapin anti-V5-tag
V5-tag Polyclonal Antibody for WB, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Protéine recombinante et plus (2)
Applications
WB, IP, IF, RIP, ChIP, ELISA
Conjugaison
Non conjugué
N° de cat : 14440-1-AP
Synonymes
"V5-tag Antibodies" Comparison
View side-by-side comparison of V5-tag antibodies from other vendors to find the one that best suits your research needs.
Applications testées
Résultats positifs en WB | Protéine recombinante |
Résultats positifs en IP | protéine protéine recombinante |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:20000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 34 publications below |
IF | See 3 publications below |
IP | See 5 publications below |
ChIP | See 1 publications below |
RIP | See 1 publications below |
Informations sur le produit
14440-1-AP cible V5-tag dans les applications de WB, IP, IF, RIP, ChIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
Réactivité | Protéine recombinante |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | V5-tag |
Masse moléculaire calculée | 1 kDa |
Symbole du gène | |
Identification du gène (NCBI) | |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.
Protocole
Product Specific Protocols | |
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WB protocol for V5-tag antibody 14440-1-AP | Download protocol |
IP protocol for V5-tag antibody 14440-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Sci Adv The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals | ||
Nat Commun Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. | ||
Nucleic Acids Res HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1. | ||
Nucleic Acids Res CGG repeats trigger translational frameshifts that generate aggregation-prone chimeric proteins. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Zhongwen (Verified Customer) (09-25-2023) | The V5 antibodys shows clear band in western blots. But if you use this antibody for IP, the background is so strong that it is hard for you to find your band.
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FH Nick (Verified Customer) (04-20-2022) | Product for IP was used and had to use above the recommended concentrations
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FH Ben (Verified Customer) (10-12-2021) | Worked well, we got good images from it even at a very low dilution
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FH Daniela (Verified Customer) (01-08-2020) | In immunoblot of HeLa total cell lysate, this antibody gives several no specific bands in the negative control sample (cell lysate from no transfected cells). Whereas it doesn't recognize any specific protein in cell lysate from HeLa transfected with a plasmid for exogenous protein in frame with V5-tag. In immunofluorescence of transfected HeLa cells this antibody give a very faint signal.
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FH Erica (Verified Customer) (12-11-2019) | I used this antibody for westtern blot of V5 tagged protein and it worked very well. The signal is very strong and there isn't background. Highly recommend
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