Anticorps Polyclonal de lapin anti-Ubiquilin 1

Ubiquilin 1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 23516-1-AP

Synonymes

DA41, DSK2, hPLIC 1, PLIC 1, PLIC1, ubiquilin 1, UBQLN1, XDRP1

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Galerie de données de validation

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  WB analysis of rat brain using 23516-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23516-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 23516-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 23516-1-AP (Ubiquilin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 23516-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 23516-1-AP (Ubiquilin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 23516-1-AP

  Immunofluorescent analysis of PC-3 cells using 23516-1-AP (Ubiquilin 1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat,
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

23516-1-AP cible Ubiquilin 1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ubiquilin 1 Protéine recombinante Ag20252
• Nom complet: ubiquilin 1
• Masse moléculaire calculée: 589 aa, 63 kDa
• Poids moléculaire observé: 63-71 kDa
• Numéro d’acquisition GenBank: BC010066
• Symbole du gène: Ubiquilin 1
• Identification du gène (NCBI): 29979
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.

Publications

Species	Application	Title
human	WB	Cancer Lett; HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1.; Authors - Zongfu Pan
human	WB	Cancers (Basel); Knockdown of UBQLN1 Functions as a Strategy to Inhibit CRC Progression through the ERK-c-Myc Pathway; Authors - Ruoxuan Ni
human	WB	Oncotarget; The mir-675-5p regulates the progression and development of pancreatic cancer via the UBQLN1-ZEB1-mir200 axis.; Authors - Jue Wang
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
human		Cancers (Basel); Development of a Novel Circulating Autoantibody Biomarker Panel for the Identification of Patients with 'Actionable' Pulmonary Nodules; Authors - Claire Auger
human	WB	Redox Biol; Inhibiting the compensatory elevation of xCT collaborates with disulfiram/copper-induced GSH consumption for cascade ferroptosis and cuproptosis; Authors - Ping Zhang