Anticorps Polyclonal de lapin anti-USP50

• Phare
• Validé par KD/KO

USP50 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20374-1-AP

Synonymes

Inactive ubiquitin carboxyl-terminal hydrolase 50, USP50

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Galerie de données de validation

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  WB analysis of mouse brain using 20374-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20374-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 20374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 20374-1-AP (USP50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cirrhose hépatique humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below

Informations sur le produit

20374-1-AP cible USP50 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ubiquitin specific peptidase 50
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: NM_203494
• Symbole du gène: USP50
• Identification du gène (NCBI): 373509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin-specific protease 50 (USP50) belongs to the peptidase C19 family. USP50 has been shown to regulate cell cycle and DNA repair through interaction with the Wee1 protein in transformed human osteosarcoma and human epithelial cell lines. USP50 has also been shown to play a role in inflammasomal function through targeting the adaptor protein (PMID: 29101126). USP50 has 2 isoforms with the molecular mass of 38 and 39 kDa. Sometimes higher molecular weight around 44 kDa can also be observed, which may be a modified variant of USP50.

Publications

Species	Application	Title
human,mouse	WB	Theranostics; Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.; Authors - Rongqing Li ; KD Validated
human,mouse	WB	EMBO Rep; Vitamin C promotes ACE2 degradation and protects against SARS-CoV-2 infection; Authors - Yibo Zuo ; KD Validated
human	WB	J Investig Med; Induction of deubiquitinating enzyme USP50 during erythropoiesis and its potential role in the regulation of Ku70 stability.; Authors - Junting Cai