Anticorps Polyclonal de lapin anti-USP37

• Phare
• Validé par KD/KO

USP37 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 18465-1-AP

Synonymes

Deubiquitinating enzyme 37, KIAA1594, Ubiquitin thiolesterase 37, USP37

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Galerie de données de validation

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  WB analysis using 18465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18465-1-AP (USP37 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 18465-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18465-1-AP (USP37 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18465-1-AP

  IP result of anti-USP37 (IP:18465-1-AP, 4ug; Detection:18465-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 18465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18465-1-AP (USP37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18465-1-AP (USP37 antibody) at dilution of 1:200 (under 1010x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18465-1-AP (USP37 antibody) at dilution of 1:200 (under 4040x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18465-1-AP (USP37 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu splénique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Informations sur le produit

18465-1-AP cible USP37 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP37 Protéine recombinante Ag12513
• Nom complet: ubiquitin specific peptidase 37
• Masse moléculaire calculée: 885 aa, 100 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC112901
• Symbole du gène: USP37
• Identification du gène (NCBI): 57695
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP37 (ubiquitin specific peptidase 37), is a member of ubiquitin-specific processing proteases family. Human USP37, localized mainly in the cytoplasm, is composed of 979 amino acids harboring three ubiquitin-interacting motifs between the Cys box and His box of the primary sequence (PMID: 27045719, 24324262). High levels of USP37 gene expression in lung cancer promoted cell viability as well as the Warburg effect via deubiquitination and stabilization of pluripotent factor c-Myc protein (PMID: 25284584). Moreover, USP37 expression was upregulated in breast cancer tissues compared with surrounding tissues and its overexpression was significantly correlated with increased rates of mortality. USP37 may represent a novel molecular target for breast cancer treatment (PMID: 30482232, 27045719).

Publications

Species	Application	Title
human	WB,IF,IHC	Nucleic Acids Res; USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM.; Authors - Chenming Wu ; KD Validated
human	WB	Proc Natl Acad Sci U S A; CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation.; Authors - Yunfan Yang
human	IF	Theranostics; PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1.; Authors - Liang Wu ; KD Validated
human	WB	Front Immunol; Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.; Authors - Wenying Gao ; KD Validated
human	WB,IHC,IF	J Exp Clin Cancer Res; PMID: 30482232; Authors - Tao Qin ; KD Validated
human	WB, IP	PLoS One; The HBx Oncoprotein of Hepatitis B Virus Deregulates the Cell Cycle by Promoting the Intracellular Accumulation and Re-Compartmentalization of the Cellular Deubiquitinase USP37.; Authors - Nehul Saxena