Anticorps Polyclonal de lapin anti-USP28

• Phare
• Validé par KD/KO

USP28 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 17707-1-AP

Synonymes

Deubiquitinating enzyme 28, KIAA1515, Ubiquitin thiolesterase 28, USP28

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Galerie de données de validation

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  WB analysis using 17707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17707-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17707-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 17707-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17707-1-AP

  IP result of anti-USP28 (IP:17707-1-AP, 4ug; Detection:17707-1-AP 1:600) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 17707-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17707-1-AP (USP28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17707-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17707-1-AP (USP28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MDA-MB-231, tissu cardiaque de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

17707-1-AP cible USP28 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP28 Protéine recombinante Ag12003
• Nom complet: ubiquitin specific peptidase 28
• Masse moléculaire calculée: 583aa,66 kDa; 1077aa,120 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC065928
• Symbole du gène: USP28
• Identification du gène (NCBI): 57646
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP28(Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).

Publications

Species	Application	Title
mouse	WB,IHC	J Clin Invest; The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.; Authors - Markus E Diefenbacher ; KD Validated
human	WB	Cell Death Differ; Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.; Authors - Yunhua Peng ; KO Validated
human	IF	EMBO J; ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.; Authors - Lauren T Evans
human	IHC	Cell Rep; The Deubiquitinase USP28 Stabilizes LSD1 and Confers Stem-Cell-like Traits to Breast Cancer Cells.; Authors - Wu Yadi Y
human	IF	J Cell Biol; A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis.; Authors - Bramwell G Lambrus
human,mouse	WB,IHC	J Biol Chem; The Deubiquitinase USP28 Maintains the Expression of the Transcription Factor MYCN and Is Essential in Neuroblastoma Cells; Authors - Junjun Li ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nikhil (Verified Customer) (11-10-2019)

Detect endogenous USP28 in A549 cell line

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: A549

USP28 Antibody Western Blot, validation (1:1500 dilution) in A549 (Cat no:17707-1-AP)