Anticorps Polyclonal de lapin anti-USP16

• Phare
• Validé par KD/KO

USP16 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14055-1-AP

Synonymes

Deubiquitinating enzyme 16, Ubiquitin thiolesterase 16, UBP M, USP16

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Galerie de données de validation

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  WB analysis using 14055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14055-1-AP (USP16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14055-1-AP

  IP result of anti-USP16 (IP:14055-1-AP, 5ug; Detection:14055-1-AP 1:1000) with HeLa cells lysate 880ug.

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  IHC staining of human liver using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14055-1-AP cible USP16 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP16 Protéine recombinante Ag4981
• Nom complet: ubiquitin specific peptidase 16
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC030777
• Symbole du gène: USP16
• Identification du gène (NCBI): 10600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP16 (Ubiquitin specific peptidase 16) was originally identified as a DUB of histone H2A which is implicated in cell cycle progression and HOXD10 gene expression (PMID:17914355). The knockout study has reported that depletion of USP16 results in early embryonic lethality in mice (PMID:24784029). Moreover, USP16 mediated the deubiquitination and stabilization of Drp1 through its direct interaction with Drp1 (PMID:37488647).

Publications

Species	Application	Title
human	WB,IF	J Cell Sci; A potent nuclear export mechanism imposes USP16 cytoplasmic localization during interphase; Authors - Nadine Sen Nkwe
mouse	WB,CoIP	Arthritis Res Ther; Deubiquitinase USP16 induces gouty arthritis via Drp1-dependent mitochondrial fission and NLRP3 inflammasome activation; Authors - Qingdong Wang ; KD Validated
human	WB,IF	J Cell Sci; A potent nuclear export mechanism imposes USP16 cytoplasmic localization during interphase.; Authors - Nadine Sen Nkwe ; KD Validated
human	IHC	Rheumatology (Oxford); Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β.; Authors - Christine Galant
human	WB,IP	Nat Commun; FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice; Authors - Gaozan Tong ; KD Validated