Anticorps Polyclonal de lapin anti-USP13

• Phare
• Validé par KD/KO

USP13 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 16840-1-AP

Synonymes

Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13

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Galerie de données de validation

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  WB analysis of HeLa using 16840-1-AP

  WB result of USP13 antibody (16840-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP13 transfected HeLa cells.

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  WB analysis using 16840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 16840-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16840-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16840-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 16840-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16840-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16840-1-AP

  WB result of USP13 antibody (16840-1-AP, 1:1000) with si-Control and si-USP13 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 16840-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16840-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16840-1-AP

  IP result of anti-USP13 (IP:16840-1-AP, 5ug; Detection:16840-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human testis using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16840-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using USP13 antibody (16840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules HEK-293, cellules PC-3, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain, tissu cérébral humain, tissu de cancer de la peau humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

16840-1-AP cible USP13 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP13 Protéine recombinante Ag10527
• Nom complet: ubiquitin specific peptidase 13 (isopeptidase T-3)
• Masse moléculaire calculée: 863 aa, 97 kDa
• Poids moléculaire observé: 97-100 kDa
• Numéro d’acquisition GenBank: BC016146
• Symbole du gène: USP13
• Identification du gène (NCBI): 8975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Genes Dev; Chaperone-mediated autophagy degrades mutant p53.; Authors - Vakifahmetoglu-Norberg Helin H
human,mouse	WB,IHC,IF	EBioMedicine; The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation.; Authors - Lian Li ; KD Validated
mouse,human	WB,IF	Transl Res; Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.; Authors - Xinxin Liao ; KO Validated
human	WB	J Cell Biol; Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death.; Authors - Hong-Guang Xia
human	WB,IP	Oncogene; USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation; Authors - Cunying Ma ; KD Validated
mouse	IP	J Cell Physiol; IL-37-induced activation of glycogen synthase kinase 3β promotes IL-1R8/Sigirr phosphorylation, internalization, and degradation in lung epithelial cells.; Authors - Lian Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Francesca (Verified Customer) (05-22-2019)

Very good antibody for western blot. Antibody specificity validated by USP13 knock-down.

• Applications: Western Blot,
• Primary Antibody Dilution: 1: 2000 in 5% milk
• Cell Tissue Type: MeT-5A cells

USP13 Antibody Western Blot, validation (1: 2000 in 5% milk dilution) in MeT-5A cells (Cat no:16840-1-AP)