Anticorps Polyclonal de lapin anti-UQCRFS1

• Phare
• Validé par KD/KO

UQCRFS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (1)

N° de cat : 18443-1-AP

Synonymes

Complex III subunit 5, Complex III subunit IX, Rieske iron sulfur protein, RIP1, RIS1, RISP, UQCR5, UQCRFS1

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Galerie de données de validation

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  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18443-1-AP

  IP result of anti-UQCRFS1 (IP:18443-1-AP, 4ug; Detection:18443-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 18443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18443-1-AP (UQCRFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 18443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18443-1-AP (UQCRFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 2 publications below

Informations sur le produit

18443-1-AP cible UQCRFS1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UQCRFS1 Protéine recombinante Ag13315
• Nom complet: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC010035
• Symbole du gène: UQCRFS1
• Identification du gène (NCBI): 7386
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. This antibody is specific to UQCRFS1.

Publications

Species	Application	Title
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
human	WB	EMBO J; EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.; Authors - Jing Zhang
pig	WB	J Cachexia Sarcopenia Muscle; MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.; Authors - Ming Qi
human,mouse	WB	Cell Biosci; Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth; Authors - Hongting Zhao
mouse	WB	J Nanobiotechnology; Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.; Authors - Li Xu
human	WB	Int J Mol Sci; Contemporary Antiretroviral Therapy Dysregulates Iron Transport and Augments Mitochondrial Dysfunction in HIV-Infected Human Microglia and Neural-Lineage Cells; Authors - Harpreet Kaur

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

ZEE (Verified Customer) (07-13-2020)

WE TRY THIS ANTIBODY WITH MOUSE HEART SAMPLES, AND GOT NICE RESULTS.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: ADULT MOUSE HEART