Anticorps Polyclonal de lapin anti-UQCRC1

• Phare
• Validé par KD/KO

UQCRC1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

36

Avis (2)

N° de cat : 21705-1-AP

Synonymes

Complex III subunit 1, Core protein I, D3S3191, QCR1, UQCR1, UQCRC1

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Galerie de données de validation

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  WB analysis using 21705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21705-1-AP

  WB result of UQCRC1 antibody (21705-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UQCRC1 transfected HeLa cells.

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  WB analysis of HeLa using 21705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

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  WB analysis of human brain using 21705-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 21705-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21705-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21705-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21705-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21705-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse large intestine using 21705-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21705-1-AP

  IP result of anti-UQCRC1 (IP:21705-1-AP, 4ug; Detection:21705-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human heart using 21705-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21705-1-AP (UQCRC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 21705-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21705-1-AP (UQCRC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 21705-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21705-1-AP (UQCRC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 21705-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21705-1-AP (UQCRC1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21705-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UQCRC1 antibody (21705-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules L02, tissu cardiaque humain, tissu cérébral humain, tissu de gros intestin de souris, tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cardiaque humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 32 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

21705-1-AP cible UQCRC1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UQCRC1 Protéine recombinante Ag16322
• Nom complet: ubiquinol-cytochrome c reductase core protein I
• Masse moléculaire calculée: 480 aa, 53 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC009586
• Symbole du gène: UQCRC1
• Identification du gène (NCBI): 7384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

Publications

Species	Application	Title
human	WB	Sci Bull (Beijing); Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects; Authors - Shuixia Tan
mouse	WB	Biomaterials; Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.; Authors - Xiao-Tao He
human	IHC	Theranostics; Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.; Authors - Qing Wang
		Acta Pharmacol Sin; MicroRNA-214 contributes to Ang II-induced cardiac hypertrophy by targeting SIRT3 to provoke mitochondrial malfunction.; Authors - Yan-Qing Ding
human	WB	Int J Nanomedicine; Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect; Authors - Wenkai Chen
human	WB	Aging (Albany NY); Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism.; Authors - Delong Yin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xingyuan (Verified Customer) (06-30-2023)

Quality was alright, had non-specific band under it.

• Applications: Western Blot
• Cell Tissue Type: 293T

UQCRC1 Antibody Western Blot validation ( dilution) in 293T (Cat no:21705-1-AP)

FH

Xingyuan (Verified Customer) (06-30-2023)

Quality was alright, had non-specific band under it.

• Applications: Western Blot
• Cell Tissue Type: 293T