Anticorps Polyclonal de lapin anti-UNC5C-Specific

UNC5C-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 20240-1-AP

Synonymes

Netrin receptor UNC5C, Protein unc 5 homolog 3, Protein unc 5 homolog C, unc 5 homolog C (C. elegans), UNC5C, UNC5C-Specific, UNC5H3

---

Galerie de données de validation

• 

  WB analysis using 20240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20240-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20240-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20240-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20240-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

20240-1-AP cible UNC5C-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-5 homolog C (C. elegans)
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 50 kDa, 103 kDa
• Numéro d’acquisition GenBank: NM_003728
• Symbole du gène: UNC5C
• Identification du gène (NCBI): 8633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UNC5C, also named as UNC5H3, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5C.

Publications

Species	Application	Title
rat	WB	Alcohol Clin Exp Res; Fetal alcohol exposure impairs learning and memory functions and elevates levels of various biochemical markers of Alzheimer's disease in the brain of twelve-month-old rats; Authors - Shaista Chaudhary
human		Sci Adv; The FLRT3-UNC5B checkpoint pathway inhibits T cell-based cancer immunotherapies; Authors - Kushal Prajapati

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Devi (Verified Customer) (02-01-2019)

20ug mouse brain cortex homogenates (lane 1 and 3: WT and Lane 2: Unc5c KO) were loaded and probed with rabbit anti-Unc5c antibody at a dilution of 1:3000 with 5% milk in TBST for 1.5 hr at room temperature. Secondary antibody was used in 1:5000 dilution and developed using ECL west femto. The only concern was that KO also showed a band albeit a faint one. WT reprised the bands showedas expected although I have concerns about bands showing up in KO tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse brain cortex homogenate

UNC5C-Specific Antibody Western Blot, validation (1:3000 dilution) in Mouse brain cortex homogenate (Cat no:20240-1-AP)