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Anticorps Polyclonal de lapin anti-UNC119

UNC119 Polyclonal Antibody for IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 13065-1-AP

Synonymes

HRG4, Protein unc 119 homolog A, Retinal protein 4, RG4, unc 119 homolog (C. elegans), UNC119

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using UNC119 Polyclonal antibody (13065-1-AP)

    IF Staining of MDCK using 13065-1-AP

    Immunofluorescent analysis of MDCK cells using 13065-1-AP (UNC119 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Applications testées

Résultats positifs en IF/ICCcellules MDCK,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

IHCSee 1 publications below

Informations sur le produit

13065-1-AP cible UNC119 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain

Réactivité canin, Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène UNC119 Protéine recombinante Ag3757
Nom complet unc-119 homolog (C. elegans)
Masse moléculaire calculée 240 aa, 27 kDa
Numéro d’acquisition GenBankBC027176
Symbole du gène UNC119
Identification du gène (NCBI) 9094
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human UNC119 protein shares strong homology with the C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. UNC119 is predominantly expressed in retina, in photoreceptor synapses and inner segments, with much lower expression in several other tissues. UNC119 is involved in the mechanism of photoreceptor neurotransmitter release through the synaptic vesicle cycle. It is required for G protein trafficking in sensory neurons.

Protocole

Product Specific Protocols
IF protocol for UNC119 antibody 13065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Ribbon Synapses and Retinal Disease: Review

Authors - Courtney E Frederick
View Article

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Francesc R (Verified Customer) (06-14-2022)

Antibody works nicely by Western blot, specifically recognizing band of roughly the expected size. Attached image shows blot of CRISPR clones: four still contain UNC119 and all the others without the band are the UNC119-KO clones.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
UNC119 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:13065-1-AP)