Anticorps Polyclonal de lapin anti-ULK1

• Phare
• Validé par KD/KO

ULK1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

58

N° de cat : 20986-1-AP

Synonymes

ATG1, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

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Galerie de données de validation

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  WB analysis of HEK-293T using 20986-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20986-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20986-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ULK1 antibody (20986-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules HEK-293T, cellules HeLa, cellules HepG2, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu de cancer du foie humain, tissu de muscle squelettique de souris, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 55 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

20986-1-AP cible ULK1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-51-like kinase 1 (C. elegans)
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 113-140 kDa
• Numéro d’acquisition GenBank: NM_003565
• Symbole du gène: ULK1
• Identification du gène (NCBI): 8408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

Publications

Species	Application	Title
human	IHC	Cancer Cell; Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis.; Authors - Hua Su
human	WB	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan ; KO Validated
human	WB	Autophagy; Topology-dependent, bifurcated mitochondrial quality control under starvation.; Authors - Yanshuang Zhou
human	IF	Dev Cell; Glucose starvation induces autophagy via ULK1-mediated activation of PIKfyve in an AMPK-dependent manner.; Authors - Cansu Karabiyik
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
mouse	WB	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane