Anticorps Polyclonal de lapin anti-UGP2

• Phare
• Validé par KD/KO

UGP2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10391-1-AP

Synonymes

pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2

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Galerie de données de validation

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  WB analysis using 10391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10391-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10391-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10391-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10391-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10391-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10391-1-AP (UGP2 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human liver cancer using 10391-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10391-1-AP (UGP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10391-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10391-1-AP (UGP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

10391-1-AP cible UGP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UGP2 Protéine recombinante Ag0590
• Nom complet: UDP-glucose pyrophosphorylase 2
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 50-56 kDa
• Numéro d’acquisition GenBank: BC002954
• Symbole du gène: UGP2
• Identification du gène (NCBI): 7360
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379)is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.

Publications

Species	Application	Title
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding
rat	IHC	Toxicol Sci; Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis.; Authors - Kakehashi Anna A
human	IHC	Dis Markers; Low UGP2 Expression Is Associated with Tumour Progression and Predicts Poor Prognosis in Hepatocellular Carcinoma.; Authors - Qiuyue Hu
human	WB	J Cell Biochem; Identification of UGP2 as a progression marker that promotes cell growth and motility in human glioma.; Authors - Chun Zeng ; KD Validated