Anticorps Polyclonal de lapin anti-UFM1

UFM1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15883-1-AP

Synonymes

BM 002, C13orf20, ubiquitin fold modifier 1, UFM1

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Galerie de données de validation

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  WB analysis of mouse lung using 15883-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis using 15883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15883-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15883-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15883-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15883-1-AP (UFM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15883-1-AP (UFM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HEK-293, cellules HepG2, cellules L02, cellules U-251, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

15883-1-AP cible UFM1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UFM1 Protéine recombinante Ag8667
• Nom complet: ubiquitin-fold modifier 1
• Masse moléculaire calculée: 85 aa, 9 kDa
• Poids moléculaire observé: 9-12 kDa
• Numéro d’acquisition GenBank: BC005193
• Symbole du gène: UFM1
• Identification du gène (NCBI): 51569
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UFM1 (Ubiquitin-fold modifier 1) is a ubiquitin-like protein covalently conjugated with intracellular proteins through UFMylation (modification by UFM1), a process similar to ubiquitylation (PMID: 38141606). UFM1 is conjugated to its target proteins by E1-like activating enzyme UBE1DC1 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation (PMID: 28234446; 33066455). At the molecular level, UFMylation is an important mediator of the protein function. Dysregulation of the UFM1 system, e.g., the knockout of UFMylation components, disturbs proteome homeostasis and triggers endoplasmic reticulum stress. Such changes are linked to developmental disorders, tumorigenesis, tissue injury, inflammation, and several hereditary neurological syndromes (PMID: 36932998). Western blot images showed that bands larger than 100kd may be proteins modified by UFM1.

Publications

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	Mol Cell Proteomics; Quantification Quality Control Emerges as A Crucial Factor to Enhance Single Cell Proteomics Data Analysis; Authors - Sung-Huan Yu
human	WB	bioRxiv; The UFMylation pathway is impaired in Alzheimer's disease; Authors - Tingxiang Yan