Anticorps Polyclonal de lapin anti-UCK2

• Phare
• Validé par KD/KO

UCK2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (2)

N° de cat : 10511-1-AP

Synonymes

Cytidine monophosphokinase 2, Testis specific protein TSA903, TSA903, UCK 2, UCK2, UK, UMPK, uridine cytidine kinase 2, Uridine monophosphokinase 2

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Galerie de données de validation

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  WB analysis of Jurkat using 10511-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10511-1-AP (UCK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10511-1-AP

  IP result of anti-UCK2 (IP:10511-1-AP, 4ug; Detection:10511-1-AP 1:500) with Jurkat cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat,
• Résultats positifs en IP: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 2 publications below

Informations sur le produit

10511-1-AP cible UCK2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCK2 Protéine recombinante Ag0782
• Nom complet: uridine-cytidine kinase 2
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC002906
• Symbole du gène: UCK2
• Identification du gène (NCBI): 7371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Uridine-cytidine kinase 2 catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively. This is the first step in the production of the pyrimidine nucleoside triphosphates required for RNA and DNA synthesis. UCK2 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.

Publications

Species	Application	Title
human	WB	Science; Oncogene-like addiction to aneuploidy in human cancers; Authors - Vishruth Girish ; KD Validated
human	WB,IHC	Cell Prolif; Prognostic and predictive role of a metabolic rate-limiting enzyme signature in hepatocellular carcinoma.; Authors - Zhangding Wang ; KD Validated
human	WB	Int J Cancer; Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.; Authors - Nao Yoshida-Sakai
human	WB	Pharmacol Res Perspect; Lack of cross-resistance to FF-10501, an inhibitor of inosine-5'-monophosphate dehydrogenase, in azacitidine-resistant cell lines selected from SKM-1 and MOLM-13 leukemia cell lines.; Authors - Motohiko Murase
human	WB	Exp Hematol; PMID: 24368162; Authors - Piyanuch Sripayap
human	WB	Cell Rep; A reversible metabolic stress-sensitive regulation of CRMP2A orchestrates EMT/stemness and increases metastatic potential in cancer.; Authors - Aristeidis E Boukouris

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Dan (Verified Customer) (05-27-2021)

Very cost effective

• Applications: Western Blot

FH

H (Verified Customer) (04-05-2020)

Primary antibody: 1:1000 dilution in 5% BSA, overnight incubation at 4CSecondary antibody: anti-rabbit IRDye800Detection: Odyssey ClxThis antibody works quite well for HEK293T and HCT116 cell lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T, HCT116

UCK2 Antibody Western Blot validation (1:1000 dilution) in HEK293T, HCT116 (Cat no:10511-1-AP)