Anticorps Polyclonal de lapin anti-UCHL5

• Phare
• Validé par KD/KO

UCHL5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11527-1-AP

Synonymes

CGI 70, INO80R, Ubiquitin thioesterase L5, UCH L5, UCH37, UCHL5

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Galerie de données de validation

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  WB analysis of mouse colon using 11527-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11527-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 11527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

11527-1-AP cible UCHL5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCHL5 Protéine recombinante Ag2095
• Nom complet: ubiquitin carboxyl-terminal hydrolase L5
• Masse moléculaire calculée: 326 aa, 37 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC025369
• Symbole du gène: UCHL5
• Identification du gène (NCBI): 51377
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).

Publications

Species	Application	Title
human	WB	Front Oncol; Ubiquitin C-Terminal Hydrolase L5 (UCHL5) Accelerates the Growth of Endometrial Cancer via Activating the Wnt/β-Catenin Signaling Pathway.; Authors - Da Liu ; KD Validated
mouse	WB	FASEB J; Caveolin-2 in association with nuclear lamina controls adipocyte hypertrophy; Authors - Moonjeong Choi
human	WB	J Cell Biochem; Computational and biochemical studies of isothiocyanates as inhibitors of proteasomal cysteine deubiquitinases in human cancer cells.; Authors - Zainab Sabry Othman Ahmed
human	WB	J Cell Biochem; Proteasome-associated cysteine deubiquitinases are molecular targets of environmental optical brightener compounds.; Authors - Isel Castro
human	IHC	Mol Ther Oncolytics; The UCHL5 inhibitor b-AP15 overcomes cisplatin resistance via suppression of cancer stemness in urothelial carcinoma; Authors - Po-Ming Chow
human	IHC	Am J Cancer Res; Anti-tumor effects of deubiquitinating enzyme inhibitor PR-619 in human chondrosarcoma through reduced cell proliferation and endoplasmic reticulum stress-related apoptosis; Authors - Wei-Chou Lin