Anticorps Polyclonal de lapin anti-UBE2S

• Phare
• Validé par KD/KO

UBE2S Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14115-1-AP

Synonymes

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

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Galerie de données de validation

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  WB analysis using 14115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14115-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 14115-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14115-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of human liver using 14115-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 14115-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14115-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293T using 14115-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  IP experiment of A2780 using 14115-1-AP

  IP Result of anti-UBE2S (IP:14115-1-AP, 4ug; Detection:14115-1-AP 1:1000) with A2780 cells lysate 960ug.

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  IHC staining of human liver cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14115-1-AP (UBE2S antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14115-1-AP (UBE2S antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 14115-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug UBE2S antibody (14115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A2780, cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules MDA-MB-453s, cellules MOLT-4, cellules PC-3, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: cellules A2780
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cutané humain, tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 3 publications below

Informations sur le produit

14115-1-AP cible UBE2S dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2S Protéine recombinante Ag5264
• Nom complet: ubiquitin-conjugating enzyme E2S
• Masse moléculaire calculée: 222 aa, 24 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC066948
• Symbole du gène: UBE2S
• Identification du gène (NCBI): 27338
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

Publications

Species	Application	Title
mouse	WB	Protein Cell; BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.; Authors - Mingzhu Wang ; KD Validated
human	WB,IHC	Int J Biol Sci; UBE2S as a novel ubiquitinated regulator of p16 and β-catenin to promote bone metastasis of prostate cancer.; Authors - Shengmeng Peng ; KD Validated
human	WB	Int J Biol Sci; The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.; Authors - Wei Xu
human	WB,IHC	J Ovarian Res; UBE2S promotes the progression and Olaparib resistance of ovarian cancer through Wnt/β-catenin signaling pathway.; Authors - Wenjing Hu
human	WB,IHC	Biochem Biophys Res Commun; UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.; Authors - Shusaku Yoshimura ; KD Validated
human	WB,CoIP	Cell Rep; Ubiquitin-Conjugating Enzyme 2S Enhances Viral Replication by Inhibiting Type I IFN Production through Recruiting USP15 to Deubiquitinate TBK1.; Authors - Li Huang ; KO Validated