Anticorps Polyclonal de lapin anti-UBE2E3

UBE2E3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15488-1-AP

Synonymes

UBCE4, UBCH9, UbcM2, UBE2E3, Ubiquitin carrier protein E3, Ubiquitin protein ligase E3

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Galerie de données de validation

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  WB analysis of Jurkat using 15488-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 15488-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15488-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15488-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15488-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 15488-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15488-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Y79, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

15488-1-AP cible UBE2E3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2E3 Protéine recombinante Ag7838
• Nom complet: ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-27 kDa
• Numéro d’acquisition GenBank: BC003554
• Symbole du gène: UBE2E3
• Identification du gène (NCBI): 10477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cells; Inhibition of miR-143-3p Restores Blood-Testis Barrier Function and Ameliorates Sertoli Cell Senescence; Authors - Ziyan Xiao
rat	WB	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.; Authors - Kazuhiko Watabe
mouse	WB	Int J Mol Sci; miR-143-3p Promotes Ovarian Granulosa Cell Senescence and Inhibits Estradiol Synthesis by Targeting UBE2E3 and LHCGR; Authors - Jingxian Deng
monkey	WB	Sci Adv; TRIM37 is a primate-specific E3 ligase for Huntingtin and accounts for the striatal degeneration in Huntington's disease; Authors - Yiyang Qin