Anticorps Polyclonal de lapin anti-UBE2A

UBE2A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 11080-1-AP

Synonymes

HHR6A, HR6A, RAD6 homolog A, RAD6A, UBC2, UBE2A, Ubiquitin carrier protein A, Ubiquitin protein ligase A

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Galerie de données de validation

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  WB analysis of HEK-293 using 11080-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11080-1-AP (UBE2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11080-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11080-1-AP (UBE2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11080-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11080-1-AP (UBE2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11080-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11080-1-AP (UBE2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human nasopharyngeal carcinoma using 11080-1-AP

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 11080-1-AP (UBE2A antibody) at dilution of 1:200 (under 40x lens)..

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules COLO 320, tissu placentaire humain, tissu rénal humain
• Résultats positifs en IHC: tissu de carcinome nasopharyngé humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

11080-1-AP cible UBE2A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2A Protéine recombinante Ag1567
• Nom complet: ubiquitin-conjugating enzyme E2A (RAD6 homolog)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC010175
• Symbole du gène: UBE2A
• Identification du gène (NCBI): 7319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE2A(Ubiquitin-conjugating enzyme E2 A) serves as the cognate E2-conjugating enzyme. It plays a critical role in regulating TP53 protein levels under both normal and stress conditions. UBE2A, UBE2B regulates TP53 levels in a "yin-yang" manner through a combination of two distinct mechanisms in mammalian cells(PMID:22083959). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	J Cell Sci; A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.; Authors - Fabienne C Fiesel
rat	WB	Mol Med Rep; Yuan‑zhi‑san inhibits tau protein aggregation in an Aβ1‑40‑induced Alzheimer's disease rat model via the ubiquitin‑proteasome system.; Authors - Bin Li
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandra (Verified Customer) (03-20-2024)

I was searching for a Rad6 antibody is strong enough to visualize endogenous protein. This is it. Works pretty good with endogenous Rad6 and extremely good with transfected cell lisate (for that I recomend a higher dilution, with 1000x the signal can be too strong)

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

UBE2A Antibody Western Blot,Immunohistochemistry,Immunofluorescence,Immunoprecipitation,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:11080-1-AP)