Anticorps Polyclonal de lapin anti-UBE1

• Phare
• Validé par KD/KO

UBE1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 15912-1-AP

Synonymes

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

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Galerie de données de validation

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  WB analysis of HL-60 using 15912-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15912-1-AP

  WB result of UBE1 antibody (15912-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE1 transfected HEK-293 cells.

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  WB analysis of mouse spleen using 15912-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15912-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 15912-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15912-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15912-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15912-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HEK-293, cellules HeLa, tissu de côlon de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below

Informations sur le produit

15912-1-AP cible UBE1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE1 Protéine recombinante Ag8703
• Nom complet: ubiquitin-like modifier activating enzyme 1
• Masse moléculaire calculée: 1058 aa, 118 kDa
• Poids moléculaire observé: 114-118 kDa
• Numéro d’acquisition GenBank: BC013041
• Symbole du gène: UBA1
• Identification du gène (NCBI): 7317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE1(Ubiquitin-activating enzyme E1) is also named as A1S9T, UBE1 and belongs to the ubiquitin-activating E1 family. It catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation and initiates the activation and conjugation of ubiquitin-like proteins. Defects in UBE1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2). UBE1 has two isoforms with the molecular mass of 118 and 114 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
mouse	IHC	ACS Nano; Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.; Authors - Yakun Wu
human		Cancer Biol Med; CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16INK4a.; Authors - Wenqi Du
human	WB	Am J Cancer Res; The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex.; Authors - Zheng Xu
human	IHC	Front Oncol; Ubiquitin-Like Modifier Activating Enzyme 1 as a Novel Diagnostic and Prognostic Indicator That Correlates With Ferroptosis and the Malignant Phenotypes of Liver Cancer Cells.; Authors - Yiru Shan
human	WB	J Mol Med (Berl); Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.; Authors - Yuhong Zhang